Photoprotection in Plants Involves a Change in Lutein 1 Binding Domain in the Major Light-harvesting Complex of Photosystem II (2024)

Ilioaia, C., Johnson, M. P., Liao, P. N., Pascal, A. A., van Grondelle, R., Walla, P. J., Ruban, A. V., & Robert, B. (2011). Photoprotection in Plants Involves a Change in Lutein 1 Binding Domain in the Major Light-harvesting Complex of Photosystem II. Journal of Biological Chemistry, 286(31), 27247-27254. https://doi.org/10.1074/jbc.M111.234617

Ilioaia, C. ; Johnson, M.P. ; Liao, P.N. et al. / Photoprotection in Plants Involves a Change in Lutein 1 Binding Domain in the Major Light-harvesting Complex of Photosystem II. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 31. pp. 27247-27254.

@article{5eb2081ce6974f548b7b76a1d4c66e3c,

title = "Photoprotection in Plants Involves a Change in Lutein 1 Binding Domain in the Major Light-harvesting Complex of Photosystem II",

abstract = "Nonphotochemical quenching (NPQ) is the fundamental process by which plants exposed to high light intensities dissipate the potentially harmful excess energy as heat. Recently, it has been shown that efficient energy dissipation can be induced in the major light-harvesting complexes of photosystem II (LHCII) in the absence of protein-protein interactions. Spectroscopic measurements on these samples (LHCII gels) in the quenched state revealed specific alterations in the absorption and circular dichroism bands assigned to neoxanthin and lutein 1 molecules. In this work, we investigate the changes in conformation of the pigments involved in NPQ using resonance Raman spectroscopy. By selective excitation we show that, as well as the twisting of neoxanthin that has been reported previously, the lutein 1 pigment also undergoes a significant change in conformation when LHCII switches to the energy dissipative state. Selective two-photon excitation of carotenoid (Car) dark states (Car S",

author = "C. Ilioaia and M.P. Johnson and P.N. Liao and A.A. Pascal and {van Grondelle}, R. and P.J. Walla and A.V. Ruban and B. Robert",

year = "2011",

doi = "10.1074/jbc.M111.234617",

language = "English",

volume = "286",

pages = "27247--27254",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "31",

}

Ilioaia, C, Johnson, MP, Liao, PN, Pascal, AA, van Grondelle, R, Walla, PJ, Ruban, AV & Robert, B 2011, 'Photoprotection in Plants Involves a Change in Lutein 1 Binding Domain in the Major Light-harvesting Complex of Photosystem II', Journal of Biological Chemistry, vol. 286, no. 31, pp. 27247-27254. https://doi.org/10.1074/jbc.M111.234617

Photoprotection in Plants Involves a Change in Lutein 1 Binding Domain in the Major Light-harvesting Complex of Photosystem II. / Ilioaia, C.; Johnson, M.P.; Liao, P.N. et al.
In: Journal of Biological Chemistry, Vol. 286, No. 31, 2011, p. 27247-27254.

Research output: Contribution to Journal › Article › Academic › peer-review

TY - JOUR

T1 - Photoprotection in Plants Involves a Change in Lutein 1 Binding Domain in the Major Light-harvesting Complex of Photosystem II

AU - Ilioaia, C.

AU - Johnson, M.P.

AU - Liao, P.N.

AU - Pascal, A.A.

AU - van Grondelle, R.

AU - Walla, P.J.

AU - Ruban, A.V.

AU - Robert, B.

PY - 2011

Y1 - 2011

N2 - Nonphotochemical quenching (NPQ) is the fundamental process by which plants exposed to high light intensities dissipate the potentially harmful excess energy as heat. Recently, it has been shown that efficient energy dissipation can be induced in the major light-harvesting complexes of photosystem II (LHCII) in the absence of protein-protein interactions. Spectroscopic measurements on these samples (LHCII gels) in the quenched state revealed specific alterations in the absorption and circular dichroism bands assigned to neoxanthin and lutein 1 molecules. In this work, we investigate the changes in conformation of the pigments involved in NPQ using resonance Raman spectroscopy. By selective excitation we show that, as well as the twisting of neoxanthin that has been reported previously, the lutein 1 pigment also undergoes a significant change in conformation when LHCII switches to the energy dissipative state. Selective two-photon excitation of carotenoid (Car) dark states (Car S

AB - Nonphotochemical quenching (NPQ) is the fundamental process by which plants exposed to high light intensities dissipate the potentially harmful excess energy as heat. Recently, it has been shown that efficient energy dissipation can be induced in the major light-harvesting complexes of photosystem II (LHCII) in the absence of protein-protein interactions. Spectroscopic measurements on these samples (LHCII gels) in the quenched state revealed specific alterations in the absorption and circular dichroism bands assigned to neoxanthin and lutein 1 molecules. In this work, we investigate the changes in conformation of the pigments involved in NPQ using resonance Raman spectroscopy. By selective excitation we show that, as well as the twisting of neoxanthin that has been reported previously, the lutein 1 pigment also undergoes a significant change in conformation when LHCII switches to the energy dissipative state. Selective two-photon excitation of carotenoid (Car) dark states (Car S

U2 - 10.1074/jbc.M111.234617

DO - 10.1074/jbc.M111.234617

M3 - Article

SN - 0021-9258

VL - 286

SP - 27247

EP - 27254

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 31

ER -

Ilioaia C, Johnson MP, Liao PN, Pascal AA, van Grondelle R, Walla PJ et al. Photoprotection in Plants Involves a Change in Lutein 1 Binding Domain in the Major Light-harvesting Complex of Photosystem II. Journal of Biological Chemistry. 2011;286(31):27247-27254. doi: 10.1074/jbc.M111.234617